Validation of Molecular Markers for Tagging the Combined Resistance for Bacterial Wilt and Tomato Leaf Curl Virus Diseases in Tomato
Keywords:
Bacterial wilt, Disease resistance, Marker Assisted Selection, Molecular breeding, ToLCV, Solanum lycopersicumAbstract
Molecular markers have been used for identification and mapping of genes and QTLs for numerous agriculturally important traits in tomato including resistance / tolerance to biotic and abiotic stresses and fruit and flower- related characteristics. Tomato leaf curl virus (ToLCV) disease caused by begomovirus and the bacterial wilt, caused by Ralstonia solanacearum are rated as the most serious production constraints in tomato. Though the breeding for disease resistance is an important objective in tomato improvement, the extent to which markers have been utilized in such programs has not been clearly determined. The utility of molecular markers for use in tomato breeding programs is limited as most of the markers reported are not validated across tomato genotypes or are not polymorphic within tomato breeding populations. In this study, the validity of available markers specific for bacterial wilt and ToLCV resistance traits in tomato was examined by testing them with an F3 population produced by crossing two resistant genotypes - Sakthi (BW resistant) and IIHR 2196 (ToLCV resistant). Most of the primers needed PCR optimization for successful amplification and some were not informative in the genotypes studied. Out of the eight markers examined, two were specific for resistance to bacterial wilt (TSCARAAG/CAT) and ToLCV (Ualty 16). Most of the available markers need to be further refined or examined for trait association and presence of polymorphism in the breeding lines and populations. However, with recent advances in tomato genome sequencing, it has become increasingly possible to develop more informative markers to accelerate the use of MAS and tagging the genes of interest in tomato breeding.Downloads
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