Evaluation of Tomato Genotypes for Tomato Leaf Curl Virus (ToLCV) Resistance
Keywords:
Tomato leaf curl virus, disease screening, genotypes, resistance sourcesAbstract
Tomato leaf curl virus disease (ToLCD), caused by whitefly transmitted begomo virus is a devastating disease affecting tomatoes worldwide. Twenty seven genotypes (Five breeding lines from World Vegetable Centre, Taiwan, nine commercial hybrids, eight germplasm accessions from NBPGR, one local collection from Idukki and four KAU varieties) were screened for ToLCV resistance under natural field conditions at Thrissur, Kerala during summer 2021. Among the screened genotypes, Ansal was found to be highly resistant, while, Kaustubh, EC 519806, Arka Rakshak and local collection (Idukki) were identified as resistant genotypes. Among the genotypes evaluated, four genotypes were moderately susceptible (AVTO 1726, AVTO 1727, Anagha and Akshaya), twelve were susceptible (AVTO 1707, AVTO 1706, AVTO 0922, Virang, Durg, Abhiraj, EC 528360, EC 620428, EC 521067 B, Manuprabha, Manulekshmi and EC 620486) and six were highly susceptible (Aryaman, Raymond, Pranay, EC 538153, EC 315489 and EC 567305) to ToLCV. The genotypes found to be highly resistant and resistant were found to be resistant to ToLCV even after whitefly mediated artificial inoculation. The resistant and highly resistant genotypes remained asymptomatic, whereas, the susceptible genotypes (Anagha and Manuprabha) exhibited ToLCV symptoms. The genotypes varied significantly with respect to plant height, number of primary branches/plant and days to flowering. Correlation between trichome density, and disease severity showed that glandular trichome density on abaxial and adaxial surfaces of leaf was significantly and negatively correlated with per cent disease incidence and disease severity index. However, the negative correlation between the abaxial glandular trichome density and ToLCV incidence was stronger. The glandular trichome density of the resistant genotypes identified in the field screening was found to be high. Non-glandular trichome density on abaxial and adaxial side of the leaf was significantly and positively correlated with per cent disease incidence and disease severity index. The resistant genotypes identified in the present study can be used for resistance breeding in tomato against ToLCV disease.References
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