Cross-genera transferability of microsatellite markers from Brassicaceae, Solanaceae and Cucurbitaceae to Momordica genus

Cross-genera transferability of microsatellite markers from Brassicaceae, Solanaceae and Cucurbitaceae to Momordica genus

Authors

  • Lavale Shivaji Ajinath Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India
  • Kelkar Vipul Ganesh Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India
  • Feba Jacob Thoppurathu Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India
  • Pradeepkumar T. College of Agriculture, Kerala Agricultural University, Thrissur 680 656, Kerala, India
  • Jiji Joseph College of Agriculture, Kerala Agricultural University, Thrissur 680 656, Kerala, India
  • Deepu Mathew Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India

Keywords:

Cucurbit, Inter-genus transferability, Microsatellites, Molecular markers, QTL

Abstract

Bitter gourd, being a rich source of phytonutrients, holds a special value in dietary habits of the consumers. Genetic improvement of this crop by inclusion of wide array of genes through inter-specific and intergeneric breeding approaches is substantially important. Microsatellites are extensively used by breeders as a molecular tool for mapping and introgression of qualitative and quantitative traits into the crop plants. This study was conducted to identify the microsatellites from Brassicaceae, Solanaceae and Cucurbitaceae family that can be efficiently used in Momordica genus. A total of 182 microsatellites from above families were studied for their amplification in two sub-species of Momordica charantia. Thirty one microsatellites were successfully amplified in bitter gourd producing 32 different alleles. The identified transferable microsatellites are useful in studying the relationships between these families and for mapping genes/QTLs in bitter gourd.

Author Biographies

Lavale Shivaji Ajinath, Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India

PhD studentCentre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur – 680 656, India

Kelkar Vipul Ganesh, Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India

PhD studentCentre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur – 680 656, India

Feba Jacob Thoppurathu, Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India

PhD studentCentre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur – 680 656, India

Pradeepkumar T., College of Agriculture, Kerala Agricultural University, Thrissur 680 656, Kerala, India

Professor and Head,Department of Vegetable Science, College of Horticulture, Kerala Agricultural University, Thrissur – 680 656, India

Jiji Joseph, College of Agriculture, Kerala Agricultural University, Thrissur 680 656, Kerala, India

Professor and Head,Department of Plant Breeding and Genetics, College of Horticulture, Kerala Agricultural University, Thrissur – 680 656, India

Deepu Mathew, Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur 680 656, Kerala, India

Assistant professor,Department of Plant Biotechnology,COH, Vellanikkara

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Published

28-07-2021

How to Cite

Ajinath, L. S., Ganesh, K. V., Thoppurathu, F. J., T., P., Joseph, J., & Mathew, D. (2021). Cross-genera transferability of microsatellite markers from Brassicaceae, Solanaceae and Cucurbitaceae to Momordica genus. Journal of Tropical Agriculture, 59(1). Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/990

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