Development and validation of a fungal spore formulation for retting of jute
Keywords:
Fungi, jute, kaolin, preservation, ready-to-use, rettingAbstract
Traditional retting of jute requires large volume of slow moving water bodies which are sometimes not available due to scarcity of rain during the harvesting season. This hampers fibre extraction from a very well grown jute crop. Fungal retting of jute is a promising technology under such condition as it requires very little water. Here we report the development and validation of a ready-to-use fungal spore formulation for retting fungi of jute. Spores of three different fungal cultures (Aspergillus flavus, A. niger and Sporotrichum thermophile designated as F1, F2 and S1, respectively) reported earlier to be suitable for dry retting of jute were preserved in kaolin powder and in a mixture of kaolin and wheat bran in different ratios for different duration up to four months. Viability of the spores, activities of retting enzymes and effectiveness of the preserved spores as inocula were studied. Spores of all the three fungal cultures were viable for at least up to 4 months with culture F2 being able to maintain maximum viability (12.77%). Solid preservative: culture ratio of 1.5:1 was found to give highest viability of F1 and F2 cultures while 2:1 was found to be the best for S1. Between kaolin and kaolin+wheat bran mix, kaolin alone was found to be better in terms of survival percentage of the fungal spores. Cultures revived after 4 months were able to carry out retting of jute under field conditions producing better quality fibre (TDN 2+50%↑) as compared to conventional water retting (TDN3+40%↑). Thus, a simple kaolin based ‘Ready-to-Use” spore formulation of 3 retting fungi was developed and validated under laboratory and field conditions.References
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