Cross-genera transferability of microsatellite markers from Brassicaceae, Solanaceae and Cucurbitaceae to Momordica genus

Lavale Shivaji Ajinath, Kelkar Vipul Ganesh, Feba Jacob Thoppurathu, Pradeepkumar T., Jiji Joseph, Deepu Mathew

Abstract


Bitter gourd, being a rich source of phytonutrients, holds a special value in dietary habits of the consumers. Genetic improvement of this crop by inclusion of wide array of genes through inter-specific and intergeneric breeding approaches is substantially important. Microsatellites are extensively used by breeders as a molecular tool for mapping and introgression of qualitative and quantitative traits into the crop plants. This study was conducted to identify the microsatellites from Brassicaceae, Solanaceae and Cucurbitaceae family that can be efficiently used in Momordica genus. A total of 182 microsatellites from above families were studied for their amplification in two sub-species of Momordica charantia. Thirty one microsatellites were successfully amplified in bitter gourd producing 32 different alleles. The identified transferable microsatellites are useful in studying the relationships between these families and for mapping genes/QTLs in bitter gourd.


Keywords


Cucurbit; Inter-genus transferability; Microsatellites; Molecular markers; QTL

Full Text:

PDF

References


Ahn, S. and Tanksley, S.D. 1993. Comparative linkage maps of rice and maize genomes. Proc. Natl. Acad. Sci., USA. 90: 7980-7984.

Azevedo, A.L.S., Costa, P.P., Machado, J.C., Machado, M.A., Vander P.A., and da Silva Ledo, F.J. 2012. Cross species amplification of Pennisetum glaucum microsatellite markers in Pennisetum purpureum and genetic diversity of napier grass accessions. Crop Science. 52(4): 1776.

Behera, T.K., Behera, S., Bharathi, L.K., John, K.J., Simon, P.W., and Staub, J.E. 2010. Bitter gourd: botany, horticulture, breeding. In: Janick, J. (ed), Horticultural Reviews, Wiley-Blackwell, pp. 101-141.

Bombonato, J.R., Bonatelli, I.A.S., Silva, G.A.R., Moraes, E.M., Zappi, D.C., Taylor, N.P., and Franco, F.F. 2019. Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae). Genetics and molecular biology. 42(1): 87-94.

Bonierbale, M.W., Plaisted, R.L., and Tanksley, S.D. 1988. RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics. 120: 1095-1103.

Chiba, N., Suwabe, K., Nunome, T. and Hirai, M., 2003. Development of microsatellite markers in melon (Cucuimis melo L.) and their application to major cucurbit crops. Breeding Sci. 53: 21-27.

Cui, J., Cheng, J., Nong, D., Peng, J., Hu, Y., He, W., Zhou, Q., Dhillon, N. P. S., and Hu, K. 2017. Genome-wide analysis of simple sequence repeats in bitter gourd (Momordica charantia). Front. Plant Sci. 8:1103.

Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. A plant DNA minipreparation: Version II. Plant Mol. Biol. Rep., 1: 19-21.

Ekue, M.R., Gailing, O. and Finkeldey, R. 2009. Transferability of simple sequence repeat (SSR) markers developed in Litchi chinensis to Blighia sapida (Sapindaceae). Plant Mol. Biol. Rep. 27(4): 570.

Grover, J. and Yadav, S. 2004. Pharmacological actions and potential uses of Momordica charantia: a review. J. Ethnopharmacol. 93(1):123-132.

Ji, Y., Luo, Y., Hou, B., Wang, W., Zhao, J., Yang, L., Xue, Q., and Ding, X. 2012. Development of polymorphic microsatellite loci in Momordica charantia (Cucurbitaceae) and their transferability to other cucurbit species. Sci. Hortic.140:115–118.

Katzir, N., Danin-Poleg, Y., Tzuri, G., Karchi, Z., Lavi, U., and Cregan, P.B. 1996. Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor. Appl. Genet. 93: 1282-1290.

Kong, Q., Xiang, C. and Yu, Z. 2006. Development of EST-SSRs in Cucumis sativus from sequence database. Mol. Ecol. Notes. 6:1234–1236.

Kong, Q., Xiang, C. and Yu, Z. 2007. Mining and charactering microsatellites in Cucumis melo expressed sequence tags from sequence database. Mol. Ecol. Notes. 7:281–283.

Kuleung, C., Baenziger, P.S. and Dweikat, I. 2004. Transferability of SSR markers among wheat, rye, and triticale. Theor. Appl. Genet., 108(6): 1147-1150.

Langercrantz, U. 1998. Comparative mapping between Arabidopsis thaliana and Brassica nigra indicates that Brassica genomes have evolved through extensive genome replication accompanied by chromosome fusion and frequent rearrangements. Genetics. 150: 1217-1228.

Lee, J.M., Nahm, S.H., Kim, Y.M., and Kim, B.D. 2004. Characterization and molecular genetic mapping of microsatellite loci in pepper. Theor. Appl. Genet., 108:619–627.

Marr, K.L., Mei, X.Y. and Bhattarai, N.K. 2004. Allozyme, morphological and nutritional analysis bearing on the domestication of Momordica charantia L. (Cucurbitaceae). Econ. Bot., 58(3):435-455.

Minamiyama, Y., Tsuro, M. and Hirai, M. 2006. An SSR-based linkage map of Capsicum annuum. Mol. Breed., 18:157–169.

Parthiban, S., Govindaraj, P. and Senthilkumar, S. 2018. Comparison of relative efficiency of genomic SSR and EST SSR markers in estimating genetic diversity in sugarcane. 3 Biotech. 8:144.

Santos, C.P., Rocha, D.S., Bajay, M.M., Santos, F.R.C., Campos, J.B., Pinheiro, J.B., Zucchi, M.I., Silva-Mann, R., Arrigoni-Blank, M.F., and Blank, A.F. 2014. Cross-species transferability of microsatellite markers in the genus Lippia. Genet. Mol. Res., 13(4): 9846-9850.

Saxena, S., Singh, A., Archak, S., Behera, T.K., John, J.K., Meshram, S.U., and Gaikwad, A.B. 2015. Development of novel simple sequence repeat markers in bitter gourd (Momordica charantia L.) through enriched genomic libraries and their utilization in analysis of genetic diversity and cross-species transferability. Appl. Biochem Biotechnol., 175(1): 93-118.

Van Wyk, B. 2015. A review of commercially important African medicinal plants. J. Ethnopharmacol., 176:118-134.

Walter, R., and Epperson, B.K. 2001. Geographic pattern of genetic variation in Pinus resinosa: Area of greatest diversity is not the origin of postglacial populations. Mol. Ecol., 10: 103-111.

Wang, S., Pan, L., Hu, K., Chen, C., and Ding, Y. 2010. Development and characterization of polymorphic microsatellite markers in Momordica charantia (Cucurbitaceae). Am. J. Bot., 97(8): e75–e78.

Wang, Z. and Xiang, C. 2013. Genetic mapping of QTLs for horticulture traits in a F2:3 population of bitter gourd (Momordica charantia L.). Euphytica. 193(2):235-250.

Wu, H., He, X., Gong, H., Luo, S., Li, M., Chen, J., Zhang, C., Yu, T., Huang, W., and Luo, J. 2016. Genetic linkage map construction and QTL analysis of two interspecific reproductive isolation traits in sponge gourd. Front. Plant Sci., 7:980.

Xin, Y., Cui, H.R., Lu, M.Z., Yao, Y.L., Jin, J.Q., Lim, Y.P., and Choi, S.Y. 2006. Data mining for SSRs in ESTs and EST-SSR marker development in Chinese cabbage. Acta Hortic. Sinica. 33:549–554.

Zhang, M., Mao, W., Zhang, G., and Wu, F. 2014. Development and characterization of polymorphic EST-SSR and genomic SSR markers for Tibetan annual wild barley. PLoS ONE. 9(4): e94881.


Refbacks

  • There are currently no refbacks.


A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]