Cross-genera transferability of microsatellite markers from Brassicaceae, Solanaceae and Cucurbitaceae to Momordica genus

Lavale Shivaji Ajinath, Kelkar Vipul Ganesh, Feba Jacob Thoppurathu, Pradeepkumar T., Jiji Joseph, Deepu Mathew


Bitter gourd, being a rich source of phytonutrients, holds a special value in dietary habits of the consumers. Genetic improvement of this crop by inclusion of wide array of genes through inter-specific and intergeneric breeding approaches is substantially important. Microsatellites are extensively used by breeders as a molecular tool for mapping and introgression of qualitative and quantitative traits into the crop plants. This study was conducted to identify the microsatellites from Brassicaceae, Solanaceae and Cucurbitaceae family that can be efficiently used in Momordica genus. A total of 182 microsatellites from above families were studied for their amplification in two sub-species of Momordica charantia. Thirty one microsatellites were successfully amplified in bitter gourd producing 32 different alleles. The identified transferable microsatellites are useful in studying the relationships between these families and for mapping genes/QTLs in bitter gourd.


Cucurbit; Inter-genus transferability; Microsatellites; Molecular markers; QTL

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