Plant regeneration of Coscinium fenestratum (Gaertn.) Colebr. through axenic seed culture and axillary bud culture

M. C. Abhaya, B. Suma

Abstract


In vitro culture methods were studied for Coscinium fenestratum (Gaertn) Colebr. (F. Menispermaceae), a critically endangered and highly traded medicinal plant, for its conservation and mass propagation. Best surface sterilization of seeds was achieved in axenic seed culture using bavistin (0.3%) for 20 min and streptocyclin (0.1%) for 20 min followed by 0.1 per cent HgCl2‚ (15 min). Among the different media tested for in vitro seed germination, the sterilized sand: coir pith (1:1) soaked with distilled water was found to be better with the highest germination percentage (22.95 %) and lowest germination time (55.68 days). An efficient shoot initiation and multiplication protocol was developed using seedling cotyledonary nodal explant in MS medium supplemented with 0.2mg L-1 BA and 0.06 mg L-1 2, 4 - D (4.5 shoots/culture). When nodal segments with an axillary bud from polyhouse grown plants were used as explants, WPMwas found to be better than the MS medium for shoot initiation and among the growth regulators, kinetin at 0.4 mg L-1 was found to be superior for shoot induction (91.63%). Zygotic embryo excised from GAƒ pre-treated seeds (4000 ppm GAƒ solution for 72 hrs.) when cultured on MS medium in dark condition for 2 weeks followed by exposure to the light condition showed faster development of the embryo, radicle emergence (100 %), plumule emergence (77.78 %) and seedling development (44.44 %).


Keywords


Conservation, Cosciniumfenestratum, Menispermaceae, micropropagation, axillary bud culture, Axenic seed culture

Full Text:

PDF PDF

References


References

Ganley, R. J. and Newcombe, G. 2006. Fungal endophytes in seeds and needles of Pinusmonticola. Mycol. Res. 110(3): 318-327.

Goveas, S. W., Madtha, R., Nivas, S. K., and D'Souza, L. 2011. Isolation of endophytic fungi from Cosciniumfenestratum- a red listed endangered medicinal plant.Eurasia J. Biosci. 5: 48-53.

Latch, G. C. M., Potter, L. R., and Tyler, B. F. 1987. Incidence of endophytes in seeds from collections of Loliumand Festuca species. Ann. Appl. boil. 111(1): 59-64.

Rojsanga, P., Suntornsuk, L., and Gritsanapan, W. 2009. Rapid Capillary Electrophoretic Analysis of Berberine in the Stem Extracts of Cosciniumfenestratum (Gaertn.) Colebr. Int. J. Biomed. Pharmaceut. Sci. 3(1): 39-41.

Senarath, W. T. P. S. K. 2010. In vitropropagatin of Cosciniumfenestratum (Gaertn.) Colebr. (Menispermaceae)-an endangered medicinal plant.J. Natn. Sci. Found. Sri Lanka. 38(4): 219-223.

Thriveni, H. N. 2015. Diversity, propagation and conservation of Cosciniumfenestratum: a critically endangered liana of the Western Ghats. Ph. D. thesis, department of applied botany, Kuvembuuniversity, Shimoga, India, 173p.

Warakagoda, P. S. and Subasinghe, S. 2014. In vitro seed germination of Cosciniumfenestratum(Gaertn.) Colebr. Annu. Res. & Rev. Biol. 4(23): 3549-3565.

Warakagoda, P. S., Subasinghe, S., and Gunasekare, M. T. K. 2017. In vitro clonal propagation of Cosciniumfenestratum (Gertn.) Colebr. (Weniwel) through nodal explants. J.Natl. Sci. Found. Sri. 45(2): 133-141.


Refbacks

  • There are currently no refbacks.


A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]