Mineral phosphate solubilization by <i>Pseudomonas aeruginosa</i> isolates from chilli (<i>Capsicum annuum</i> L.) fields
Keywords:
Gluconic acid, High performance liquid chromatography, Mineralization, Solubilization efficiency, Vigour indexAbstract
Phosphorus (P) is the second imperative key element after nitrogen as a mineral nutrient in terms of quantitative plant requirement. An adequate supply of P is obligatory for proper execution of various metabolic activities of plants. Identification of a potent phosphate solubilizing microorganism capable of transforming insoluble P into soluble and plant accessible forms is considered as the best eco-friendly option for providing inexpensive P to plants. In this study 12 phosphate solubilizing microorganisms were isolated from chilli rhizosphere by enrichment culture technique. P solubilisation efficiency was checked using vanadomolybdate phosphoric yellow colour method and two most efficient isolates, PS 2 and PS 3, were selected. Various parameters such as pH, temperature of incubation, carbon source, nitrogen source, NaCl concentration and incubation time were optimized for the selected isolates, Pseudomonas aeruginosa PS 2 (KR270346) and Pseudomonas aeruginosa PS 3 (KR270347). Maximum P solubilization rate was shown at pH 7, temperature 30°C and after 15 days of incubation. Glucose and ammonium sulphate were the best carbon and nitrogen sources for the selected isolates. Mineral phosphate solubilization was directly related to a pH drop in the culture medium. Production of gluconic acid by Pseudomonas aeruginosa during phosphate solubilization was confirmed by analyzing the culture medium by high performance liquid chromatography. The growth stimulation effect of selected isolates was confirmed by the vigour index determination and the treated rice seeds showed better germination percentage than control seeds.References
Abdul- Baki, A.and Anderson, J.D. 1973. Vigor determination in Soybean seed by multiple criteria. Crop Sci. 13, 630-633.
Chandrashekhara, Niranjanraj, S., Saligrama, A., Deepak, N., Nandini, P. and Hunthrike, S., 2007. Endophytic bacteria from different plant origin enhance growth and induce downy mildew resistance in pearl millet Asian J Plant Pathol. 1: 1-11.
Chen, Y.P., Rekha, P.D., Arun, A.B., Shen, F.T., Lai, W.A. and Young, C.C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl. Soil Ecol. 34:33-41.
Deepshikha, T., Mohinder, K. and Vineet, S. 2014. Optimization of best cultural conditions for high production of phosphate solubilizing activity by fluorescent Pseudomonas isolated from normal and replant sites of apple and pear. The Bioscan., 9(1):143-150.
Dubey, S.K. and Billore, S.D. 1992. Phosphate solubilizing microorganisms (PSM) as inoculants and their role in augmenting crop productivity in India.-a review. Crop res. 5:11-24.
Goldstein, A. H. 1994. Involvement of the quinoprotein glucose dehydrogenase in the solubilization of exogenous phosphates by gram-negative bacteria. In: Torriani-Gorini, A., Yagil, E., Silver, S., editors. Phosphate in Microorganisms: Cellular and Molecular Biology. Washington, DC: ASM Press, pp. 197–203.
Gulati, A., Rahi, P. and Vyas, P. 2007. Characterization of phosphate solubilization fluorescent pseudomonads from the rhizosphere of seabuckthorn growing in the cold desert of Himalayas. Curr. Microbiol. 56: 73-79.
Gyaneshwar, P., Naresh, K.G., Parekh, L.J. and Poole, P.S. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant Soil. 245:83–93.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staloj, J.T. and Williams, S.T. 1994. Bergey’s Manual of Determinative Bacteriology- 9th Ed. Williams and Wilkins. Baltimore/London.
Hsu, Jason . 1996. Multiple Comparisons Theory and Methods, Chapman & Hall/CRC: Boca Raton. Pp. 1-296.
Jackson, M.L. (1973). Soil chemical analysis. Prentice Hall, New Delhi, India.
Jena, S.K. and Chandi, C.R. 2013. Optimization of culture conditions of phosphate solubilizing activity of bacterial sp. isolated from Similipal biosphere reserve in solid-state cultivation by response surface methodology. Int J Curr Microbiol App Sci . 2(5):47-59.
Karpagam, T. and Nagalakshmi, P. K. 2014. Isolation and characterization of Phosphate solubilizing microbes from agricultural soil. Int J Curr Microbiol App Sci . 3(3): 601-614.
Khan, A.A., Jilani, G., Akhtar, M.S., Naqvi, S.M.S. and Rasheed, M. 2009. Phosphorus solubilizing bacteria: occurrence, mechanisms and their role in crop production. J. Ag. & Bio. Sci. 1(1):48–58.
Kuheli, D., Vandana, K. and Reeta, G. 2003. P solubilization potential of plant growth promoting Pseudomonas mutants at low temperature. Microbiol. Res. 158: 59-362.
Kumari, P.P. and Gupta, P.C. 2013. Effect of different carbon and nitrogen sources on solubilization of insoluble inorganic phosphate by psychrotolerant bacterial strains. Bioscan., 8:1299-1302.
Landweert, R., Hooffland, E., Finlay, R.D., Kuyper, T.W. and Van breemen, N. 2001. Linking plants to rocks: ectomycorrhizal fungi mobilize nutrients from minerals. Trends Ecol. Evol. 16: 248–254.
Mehta, S. and Nautiyal, C.S. 2001. An efficient method for qualitative screening of phosphate solubilizing bacteria. Curr Microbiol. 43:51-56.
Moumita, D., Rakhi, P., Chandan, S., Manas Kumar, P. and Samiran, B. 2011. Plant growth promoting rhizobacteria enhance growth and yield of chilli (Capsicum annuum L.) under field conditions. AJCS. 5(5):531-536.
Narveer Asish, V., Harsh, K. and Chayanika, P. 2014. In vitro Phosphate Solubilization by Bacillus sp. NPSBS 3.2.2 Obtained from the Cotton Plant Rhizosphere. Biosci Biotechnol Res Asia. 11(2):401-406.
Pikovskaya, R.I. 1948. Mobilization phosphorus in soil in connection with vital activity of some microbial species. Microbiologiya. 17:362-370.
Qurban, M.A., Balala, A.C., Kumar, S., Bhavya, P.S. and Wafar, M. 2014. Primary production in the northern Red Sea. Journal of Marine Systems. 132:75-82.
Rodriguez, H. and Fraga, R.1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances. 17:319-339.
Sagoe, C.I., Ando, T., Kouno, K. and Nagaoka, T. 1998. Relative importance of protons and solution calcium concentration in phosphate rock dissolution by organic acids. Soil science and plant nutrition. 44:617-625.
Sahu, M. K., Sivakumar, K. and Kannan, L. 2007. Phosphate solubilizing actinomycetes in the estuarine environment: An inventory. Journal of Environmental Biology. 28:795-798.
Srivastav, S., Yadav, K.S., and Kundu, B.S. (2004). Prospects of using phosphate solubilizing Pseudomonas as biofungicide. Indian Journal of Microbiology, 44(2):91-94.
Srividya, S., Soumya, S. and Pooja, K. 2009. Influence of environmental factors and salinity on phosphate solubilization by a newly isolated Aspergillus niger F7 from Agricultural Soil. African Journal of Biotechnology. 8:1864-1870.
Stephen, J., Shabanamol, S., Rishad, K.S. and Jisha, M.S. 2015. Growth enhancement of rice (Oryza sativa) by phosphate solubilizing Gluconacetobacter sp. (MTCC 8368) and Burkholderia sp. (MTCC 8369) under greenhouse conditions. 3 Biotech. 5(5):831–837.
Stephen, J. and Jisha, M.S. 2011. Gluconic acid production as the principal mechanism of mineral phosphate solubilization by Burkholderia sp. (MTCC 8369). Journal of Tropical Agriculture. 49 (1-2):99-103.
Vassilev, N., Baca, M.T., Vassileva, M., Franco, I., Azcon, R. and De Nobili, M. 1996. Mineralization of three agro-industrial wastes by an acid producing strain of Aspergillusniger. In The Science of Composting (Springer). 1376-1379.
Vishal Kumar, D. and Punkaj Kumar. 2013. Production of Plant growth promoting substance by Pseudomonads. Journal of Academia and Industrial Research. 2(4):221-225.
Zhu, F., Qu, L., Hong, X. and Sun, X. 2011. Isolation and characterization of a phosphate solubilizing halophilic bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the coast of Yellow Sea of China. Evidence-Based Complementary and Alternative Medicine. 2011: 615032. doi: 10.1155/2011/615032
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