Evaluation of cocoa varieties for shoot induction under in vitro condition

S. Sreelekshmi, B. Suma, J.S. Minimol, L. Joseph


In vitro propagation in cocoa is a potential tool for the rapid multiplication of true to type plants. Single noded cuttings of 2-3 cm collected from the budded plants maintained in polyhouse were used as explants. The experiment was done in five varieties: CCRP 2, CCRP 6, CCRP 8, CCRP 15 and Scavina. The best surface sterilization of explants was achieved using Mancozeb 0.2 per cent for 30 minutes followed by Cefotxim 0.1% for 10 minutes and then ethyl alcohol 70 per cent for 3 minutes and mercuric chloride 0.1 per cent for 5 minutes. Axillary bud sprouting was induced in basal Woody Plant Media (WPM) fortified with 2- isopentinyl adenine (2ip) and Indole-3-Acetic Acid (IAA). The period of single shoot induction from leaf axils was lowest (8.41) in variety CCRP 15 in media WPM+2ip 1ppm+IAA 0.02ppm. Among the varieties, highest multiple shoot induction percentage (93.75%) was recorded in variety Scavina.


Cocoa, Indole Acetic Acid, in vitro, propagation, Woody Plant Medium, 2-Isopentanyl Adenine.

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Bindu, M.R. 1997. Standardisation of in vitro techniques for rooting, hardening and micrografting in cocoa (Theobroma cacao L.). Ph. D. (Ag) thesis, Kerala Agricultural University, Thrissur, 135p.

Bonga, J.M. 1982. Vegetative propagation in relation to juvenility, maturity and rejuvenation. Tissue Culture in Forestry (eds. Bonga, J.M. and Durzan, D.J.). MartinusNijhoff, TheHague, Netherlands, pp. 387-412

Giridhar, P., Reddy, B. O., and Ravishankar, G. A. 2005. Silver nitrate influences in vitro shoot multiplication and root formation in Vanilla planifoliaAndr. Curr. Sci. 81(9): 1166-1170.

Devi, B.I. 2011. In vitro propagation in ashoka(Saracaasoca(roxb.) de wilde). MSc (Ag) thesis, Kerala Agricultural University, Thrissur, 53p.

Mallika, V.K., Sankar, A.M., Sindhu, K., Rekha, C., Vijayakumar, N.K. and Nair, R.V. 1992. Plantlet regeneration in vitro from nodal segments of cocoa (Theobroma cacao L.) andfield planting. J. Plantn. Crops. 20: 114-122.


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