Seed biopriming and spraying at fruit set with microbial agents suppress anthracnose disease and improve growth and yield in chilli

P. V. Athira, N. V. Radhakrishnan, K. N. Anith

Abstract


Anthracnose caused by Colletotrichum capsici (Syd.) Butler and Bisby is a major disease of chilli (Capsicum annuum L.) resulting in a yield loss from 8 to 60 percent in India. Most of the commercial chilli varieties are susceptible to the disease. Present study reports potential of seed biopriming and spraying with biocontrol agents at fruit-set stage for the management of anthracnose disease in chilli (var.Vellayani Athulya). Three biocontrol agents viz., Trichoderma asperellum, Bacillus subtilis VLY 62 and Bacillus amyloliquefaciens VLY 24 were compared with chemical check (Carbendazim 0.1%) and untreated control for disease management and growth promotion. Seed biopriming and spraying with B. amyloliquefaciens at a concentration of 108 cfu mL-1 was the most effective treatment in reducing the disease. Biopriming alonealso exhibited significant reduction.


Keywords


Chilli anthracnose; Colletotrichum capsici; Biopriming; Bacillus spp.; Trichoderma viride

Full Text:

PDF

References


Abarna, T., Raheem, A. H. A., Livingstone, R. A., Abichandra, K., Abikannan, A. and Raj, T.S. 2019. Bio efficacy of Bacillus subtilis and plant extracts against Colletotrichum capsici (Syd.) Butler and Bisby under in vitro condition. J. Appl. Sci. Computations 6(4): 2110-2123.

Abdul-Baki, A. A. and Anderson J. D. 1973. Vigour determination in soybean seed by multiple criteria. Crop Sci. 13: 630-633.

Amaresan, N., Jayakumar, V. and Thajuddin, N. 2014. Isolation and characterization of endophytic bacteria associated with chilli (Capsicum annuum) grown in coastal agricultural ecosystem. Indian J. Biotechnol. 13: 247-255.

Amaresan, N., Jayakumar, V., Kumar, K. and Thajuddin, N. 2012. Isolation and characterization of plant growth promoting endophytic bacteria and their effect on tomato (Lycopersicon esculentum) and chilli (Capsicum annuum) seedling growth. Ann. Microbiol. 62: 805-810.

Anand, T. and Bhaskaran, R. 2009. Exploitation of plant products and bioagents for eco-friendly management of chilli fruit rot disease. J. Plant Prot. Res. 49(2): 195-203.

Ananthi, M., Selvaraju, P. and Sundaralingam, K. 2017. Standardization of suitable concentration and duration of seed biopriming with humic acid in chilli. Int. J. Chem. Studies 5(3): 636-639.

Arrebola, E., Sivakumar, D., Bacigalupo, R. and Korsten, L. 2010. Combined application of antagonist Bacillus amyloliquefaciens and essential oils for the control of peach postharvest diseases. Crop Prot. 29: 369-377.

Asalmol, M. N., Kale, V. P. and Ingle, S. T. 2001. Seed borne fungi of chilli, incidence and effect on seed germination. Seed Res. 29(1): 76-79.

Athira, S. and Anith, K.N., 2020. Plant growth promotion and suppression of bacterial wilt incidence in tomato by rhizobacteria, bacterial endophytes and the root endophytic fungus Piriformospora indica. Indian Phytopathol. 73(4): 629-642.

Bais, H. P., Fall, R. and Vivanco, J. M. 2004. Biocontrol of Bacillus subtilis against infection of Arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiol. 134: 307–319.

Bankole, S. A. and Adebanjo, A. 1996. Biocontrol of brown blotch of cowpea caused by Colletotrichum truncatum with Trichoderma viride. Crop. Prot. 15(7): 633-636.

Bharathi, R., Vivekananthan, R., Harish, S., Ramanathan, A. and Samiyappan, R. 2004. Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Prot. 23: 835-843.

Callan, N. W., Mathre, D. E. and Miller, J. B. 1991. Field performance of sweet corn seed bioprimed and coated with Pseudomonas fluorescens ab254. Hort. Sci. 26: 1163-1165.

Chauhan, R. and Patel, P. R. 2017. Evaluation of seed bio-priming against chilli (Capsicum frutescence L.) cv. GVC 111 in vivo. J. Pharmacog. Phytochem. 6(6): 176-181.

Christopher, D. J., Raj, T. S. and Kumar, R. S. R. 2014. Induction of defence enzymes activity in chilli against Colletotrichum capsici using IDM tools. Indian J. Plant Prot. 42(3): 242-247.

Debergh, P. C., De Coster, G. and Steurbaut, W. 1993. Carbendazim as an alternative plant growth regulator in tissue culture systems. In Vitro Cell Dev. Biol. 29: 89-91.

Dhanalakshmi, G. 2013. Biopriming studies using biocontrol agents and liquid biofertilizers in tomato and chilli seed. M.Sc. (Ag.) Thesis, Tamil Nadu Agricultural University, Coimbatore, 274p.

El-Mohamedy, R. S. R. 2004. Biopriming of okra seed to control damping off and root rot diseases. Annals Agric. Sci. 49(1): 339-356.

El-Mougy, N. S. and Abdel-Kader, M. M. 2008. Long-term activity of bio-priming seed treatment for niological control of faba bean root rot pathogens. Australasian Plant Pathol. 37(5): 464-471.

Geetha, R. and Selvarani, K. 2017. A study of chilli production and export from India. Int. J. Adv. Res. Innovative Ideas Edu. 3(2): 205-210.

Ghassemi, G. K., Sheikhzadeh, M. P. and Valizadeh, M. 2008. Effect of hydropriming duration and limited irrigation on field performance of chickpea. Res. J. Seed Sci. 1(1): 34-40.

Gowtham, H. G., Murali, M., Singh, S. B., Lakshmeesha, T. R., Murthy, K. N., Amruthesh, K. N. and Niranjana, S. R. 2018. Plant growth promoting rhizobacteria – Bacillus amyloliquefaciens improves plant growth and induced resistance in chilli against anthracnose disease. Biol. Control. 126: 209-217.

Hernandez, S. C., Perez, J. J. R. Contreras, R. G. C. Enrquez, G. R. Cabrera, C. R. C. and Montiel. L. G. H. 2019. Biocontrol of postharvest fruit fungal diseases by bacterial antagonists: a review. Agronomy 9(121): 101-115.

Howard, L. R., Talcott, S. T. Brenes, C. H. and Villalon, B. 2000. Changes in phytochemical and antioxidant activity of selected pepper cultivars (Capsicum spp.) as influenced by maturity. J. Agric. Food Chem. 48 (5): 1713-1720.

Ilyas, S., Asie, K. V., Sutariati, G. A. K and Sudarsono. 2015. Biomatriconditioning or biopriming with biofungicides or biological agents applied on hot pepper (Capsicum annuum L.) seed reduced seed borne Colletotrichum capsici and increased seed quality and yield. Acta Hortic. 1105: 89-96.

Inglis, D. A., Haedorn, D. J. and Rand, R. E. 1988. Use of dry inoculum to evaluate beans for resistance to anthracnose and angular leaf spot. Plant Dis. 72: 771-774.

Ippolito, A. and Nigro, F. 2000. Impact of pre-harvest application of biological control agents on postharvest diseases of fresh fruit and vegetables. Crop Prot. 19 (10): 715-723.

ISTA [International Seed Testing Association]. 1985. International rules for seed testing. Seed Sci. Tech. 13(2): 229-355.

Jayapala, N., Mallikarjunaiah, H., Puttaswamy, H., Gavirangappa, H. and Ramachandrappa, N. S. 2019. Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chilli (Capsicum annuum L.) through activating host defense response. Egyptian J. Biol. Pest Control. 29: 45-54.

Lamsal, K., Kim, S. W., Kim, Y. S. and Lee, Y. S. 2012. Application of rhizobacteria for plant growth promotion effect and biocontrol of anthracnose caused by Colletotrichum acutatum on pepper. Mycobiol. 40(4): 244-251.

Machenahalli, S., Nargund, V. B. and Hegde. R. V. 2014. Management of fruit rot causing seed borne fungal pathogens in chilli. Bioscan. 9(1): 403-406.

Meena, Y. K., Singh, Y. and Kharayat, B. S. 2012. Biological control of anthracnose of sorghum caused by Colletotrichum graminicola. Int. J. Plant Prot. 5(2): 333-338.

Mishra, B. K., Mishra, R. K., Mishra, R. C., Tiwari, A. K., Yadav, R. S. and Dikshit, A. 2011. Biocontrol efficacy of T. viride isolates against fungal plant pathogens causing disease in Vigna radiata L. Arch. Appl. Sci. Res. 3(2): 361-369.

Naik, M. 2015. Effect of biopriming on seed quality and management of seed borne pathogens in garden pea (Pisum sativum L.). M.Sc. (Ag) thesis, Orissa University of Agriculture and Technology, 66p.

Pooja, G. and Simon, S. 2019. Integrated approaches for management of anthracnose of chilli (Capsicum annuum L.). J. Pharmacogn. Phytochem. 8(1): 422-427.

Rai, A. K. and Behera. S.2019. Response of seed bio-priming on chilli (Capsicum annuum L.). J. Pharmacog. Phytochem. 8(1): 900-903.

Sahitya, U. L., Sri Deepthi, R. and Krishna, M. 2014. Anthracnose, a prevalent disease in capsicum. Res. J. Pharma. Biol. Chem. Sci. 5(3): 1583-1604.

Turner, J. T. and Backman, P. A. 1991. Factors relating to peanut yield increases after seed treatment with Bacillus subtilis. Plant Dis. 75:347–353.

Vishwakarma, S. N. and Sitaramaiah, K. 1986. Relative efficacy of fungicides in field for the control of dieback and fruit rot of chilli (Capsicum annuum L.). Adv. Bio. Res. 4: 128-137.

Yamamoto, S., Shiraishi, S. and Suzuki, S. 2014. Are cyclic lipopeptides produced by B. amyloliquefaciens S13-3 responsible for the plant defence response in strawberry against C. gloeosporioides. Appl. Microbiol. 60(4): 379-386.


Refbacks

  • There are currently no refbacks.


A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]