Assessment of diversity in <i>Garcinia indica</i> (Dupetit-Thouars.) Choisy. using morphological and molecular markers

Assessment of diversity in <i>Garcinia indica</i> (Dupetit-Thouars.) Choisy. using morphological and molecular markers

Authors

  • Ketan S. Thatte Kelkar Education Trust’s V.G.Vaze College of Arts, Science & Commerce, Department of Botany, Mithagar Road, Mulund (E.), Mumbai, Maharashtra, India
  • R. G. Khandekar Department of Horticulture, Dr. Balasaheb Sawant Agricultural University, Dapoli, District Ratnagiri, Maharashtra, India
  • Manjushree A. Deodhar Kelkar Education Trust’s V.G.Vaze College of Arts, Science & Commerce, Department of Botany, Mithagar Road, Mulund (E.), Mumbai, Maharashtra, India

Keywords:

Endemism, Inter and intra-population variability, Germplasm conservation

Abstract

Garcinia indica (Dupetit-Thouars.) Choisy. is an endemic plant in the southern Western Ghats. Although endemic species show low levels of genetic diversity, morphological variations abound in G. indica. Genetic diversity of G. indica plants collected from four locations in Maharashtra were evaluated at inter and intra-population levels using morphological traits and molecular markers. Leaf length and plant height showed profound variability. Plants exhibited less diversity within the population when screened by molecular markers like RAPD and ISSR. In case of RAPD primers, the highest diversity was observed for the Sawantwadi population (19.93%), intermediate variation in Diveagar (8.11%), low variation in Chiplun (5.20%), and least variability in Dapoli populations (4.10%). Similarly, 10 ISSR primers expressed 11.37% variation in Sawantwadi region, 9.20% in Dive-agar, 4.70% in Dapoli, and only 1.20% in Chiplun. There were small pockets, e.g., Dive-agar or Otavane in Sawantwadi in which the plants showed diversity at both morphological as well as molecular levels, implying the need to conserve the germplasm of these populations.

Author Biographies

Ketan S. Thatte, Kelkar Education Trust’s V.G.Vaze College of Arts, Science & Commerce, Department of Botany, Mithagar Road, Mulund (E.), Mumbai, Maharashtra, India

Lecturer, Dept. of Biotechnology, KET"s V. G. Vaze College, Mulund (E.), Mumbai-81

Manjushree A. Deodhar, Kelkar Education Trust’s V.G.Vaze College of Arts, Science & Commerce, Department of Botany, Mithagar Road, Mulund (E.), Mumbai, Maharashtra, India

Associate professor, Dept. of Botany, KET"s V. G. Vaze College, Mulund (E.), Mumbai-81

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Published

17-10-2012

How to Cite

Thatte, K. S., Khandekar, R. G., & Deodhar, M. A. (2012). Assessment of diversity in <i>Garcinia indica</i> (Dupetit-Thouars.) Choisy. using morphological and molecular markers. Journal of Tropical Agriculture, 50(1), 30–36. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/263

Issue

Vol. 50 No. 1 (2012)

Section

Regular papers

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