Comparative evaluation of various extraction methods on quantity and quality of Indigo dye from Indigofera tinctoria L.

Comparative evaluation of various extraction methods on quantity and quality of Indigo dye from Indigofera tinctoria L.


  • Sekar Kaliaperumal Annamalai University, Annamalai nagar-608002, Tamil Nadu, India


Natural Dyes, Indigo, Extraction methods


An attempt was made to standardize the extraction of indigo from Indigofera tinctoria L. for maximizing the dye recovery and maintaining quality through various extraction methods. The experiment consisting factorial combinations of four levels of method of extraction viz., conventional,microbial , chemical methods and hot water treatment and seven levels of duration of fermentation viz., 0, 4, 8, 12, and 16, 20 and 24 h. The method of extraction showed significant influence on indigo content and dye yield. The highest dye recovery and indigo content were recorded with microbial fermentation method. The microbial method not only increased the yield and quality but also reduced the duration of extraction.


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How to Cite

Dhanraj, D., & Kaliaperumal, S. (2022). Comparative evaluation of various extraction methods on quantity and quality of Indigo dye from Indigofera tinctoria L. Journal of Tropical Agriculture, 59(2). Retrieved from




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