RAPD profile based grouping of garlic <i>Allium sativum</i> germplasm with respect to photoperiodism
Keywords:
Molecular markers, Genotypes, Genetic diversityAbstract
Forty garlic genotypes were evaluated using RAPD markers to determine the genetic relationships among genotypes showing morphological variations. Eighteen decamer primers were used, all of which were polymorphic in the set of cultivars studied and allowed every genotype to be unambiguously distinguished. Genetic diversity in the population studied was analyzed using several variability parameters. A dendrogram based on UPGMA analysis grouped the 40 genotypes into three main clusters with Jaccard’s similarity coefficient ranging from 0.00 to 0.75 with an average of 0.25. Cluster I represented short day garlic genotypes, cluster II day-neutral garlic selections, and cluster III long day garlic selections. Principal Coordinate (PCO) analysis was comparable to the results of cluster analysis. RAPD marker based grouping of garlic genotypes with respect to photoperiod requirement will be useful in garlic improvement.Downloads
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