Development of protocorm-like bodies and shoots in <i>Dendrobium</i> cv. Sonia following gamma irradiation
Keywords:
In vitro mutagenesis, Additive effectsAbstract
This paper reports a preliminary attempt to evolve mutant varieties of Dendrobium with desirable traits. Dendrobium cv. Sonia was subcultured on half-strength MS medium supplemented with BA 1.0 mg L-1 and NAA 1.0 mg L-1 to induce production of protocorm-like bodies (PLBs). The PLBs were exposed to different doses of gamma rays (20 Gy and 30 Gy) and re-irradiated from a cobalt 60 source. The highest number of shoots was observed at 20 Gy (3.4). A comparison of three levels of BA with ½ MS media on the irradiated protocorms showed more PLB production at 0.5 mg L-1 while shoot production was higher at 1.5 mg L-1.Downloads
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