Priming impacts the germination and biochemical parameters of seeds in bitter gourd (Momordica charantia L.)
Abstract
Seeds of bitter gourd variety Preethi stored under ambient environment were subjected to various priming treatments after six months of storage aiming to improve germination.The eleven priming treatments employed were T1 : KNO3 0.3 % for 2 h, T2 : KNO3 0.015 % for 3 h, T3 : KH2 P04 0.01 M for 24 h, T4 : PEG 6000 -1.5 MPa for 24 h, T5 : GA3 100 ppm for 24 h, T6 : Solid matrix priming with Perlite for 48 h, T7 : Solid matrix priming with Cocopeat for 48 h, T8 : Psuedomonas fluorescens 1x106 cfu.ml- ¹ for 24 h, T9 : Hydration hot water at 50° C for 4 h, T10: Hydration with cold water at 28o C for 24 h and T11:Absolute control. The results indicated that solid matrix priming with perlite for 48 hours significantly enhanced germination parameters and seed biochemical indices in bitter gourd (Momordica charantia) seeds. Solid matrix priming with cocopeat for 48 hours and seed treatment with Pseudomonas fluorescens (1×10^6 cfu.ml- ¹) were also effective, though to a slightly lesser extent in a few instances than perlite-based priming. Osmopriming with PEG 6000 (-1.5 MPa for 24 h) was found to be detrimental and found to be inferior to untreated control.
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