Detection of <i>Ralstonia solanacearum</i> race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis

Detection of <i>Ralstonia solanacearum</i> race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis

Authors

  • Deepa James College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala
  • D. Girija College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala
  • Sally K. Mathew College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala
  • P.A. Nazeem College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala
  • T.D. Babu College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala
  • A. Sukumara Varma College of Horticulture, Kerala Agricultural University, Thrissur 680 656, Kerala

Keywords:

Bacterial wilt, biovar, race, Ralstonia solanacearum, RAPD, solanaceous vegetables

Abstract

Nine strains of Ralstonia solanacearum (Smith) Yabuuchi et al. isolated from bacterial wilt affected plants of brinjal, chilli and tomato in three different agroclimatic zones of Kerala were compared based on the utilization of carbohydrates, hypersensitivity reaction on capsicum leaves and RAPD analysis. Among these, six isolates were grouped into Biovar III and three, into Biovar IIIA. The isolates belonged to Races 1 and 3. RAPD analysis with 10 decamer primers revealed a high degree of polymorphism among the isolates. The primer OPF 8 yielded a unique band of 1.45 kb size for Race 3. This could be considered as a marker for rapid identification of Race 3 isolates of R. solanacearum.

Downloads

Published

27-10-2006

How to Cite

James, D., Girija, D., Mathew, S. K., Nazeem, P., Babu, T., & Varma, A. S. (2006). Detection of <i>Ralstonia solanacearum</i> race 3 causing bacterial wilt of solanaceous vegetables in Kerala, using random amplified polymorphic DNA (RAPD) analysis. Journal of Tropical Agriculture, 41, 33–37. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/94

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

1 2 3 4 5 6 > >> 

You may also start an advanced similarity search for this article.

Loading...