Induction of multiple shoots through in vitro male bud culture in banana Musa (AA) cv. ‘Kadali’

K. S. Lakshmi, Jyothi Bhaskar, A. Suma, P. B. Pushpalatha

Abstract


Male buds of banana (Musa (AA) cv. ‘Kadali’) collected 20-25 days after the emergence of bunches were used for in vitro shoot regeneration, during 2016-2018. Sterilized male buds were inoculated on Murashige and Skoog basal medium with different combinations of naphthalene acetic acid (NAA) and benzyl adenine (BA). The earliest and highest percentage of culture establishment and multiple shoot induction (15.99) were observed on full strength MS medium with NAA 1.0 mgL-1 and BA 4.0 mgL-1.


Keywords


Kadali, Musa, Male bud, In vitro propagation, Direct organogenesis, MS media, Benzyl adenine, Naphthalene acetic acid

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]