Molecular and field level screening for blast resistance gene donors among traditional rice varieties of Kerala

Henry Nickolas, V.G . Jayalekshmy, C.K. Yamini Varma, Veena Vighneswaran

Abstract


Rice blast, caused by Magnaporthe oryzae, is becoming one of the most devastating diseases of rice (Oryza sativa L.) in Kerala. Use of resistant rice varieties had been proved to be the most effective and sustainableway to control this disease. In the present study, the existence of blast resistance genes Pi 1 , Pi 2 and Pi kh in selected landraces of Kerala were identified using the SSR markers RM224, RM527 and RM206 respectively, and further confirmed through a field level screening. Results showed the presence of gene Pi1 in nine varieties, Pi 2 in twenty varieties and Pi kh in six varieties. Infection index analysis after field levelscreening showed that nine varieties had low infection index. Landraces carrying the genes Pi 1 and Pi kh imparted moderate resistance under Kerala conditions which implied that pyramiding of these genes could improve the disease resistance in rice varieties of Kerala. Among the selected varieties, Parambuvattan and Kavunginpoothala, having both Pi 1 and Pi kh genes could be used as gene donors in further breeding programmes.


Keywords


Traditional varieties; Rice blast; Disease Resistance; SSR markers; Field screening

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References


Aneja, K. 2009. 'Experiments in microbiology, plant pathology and biotechnology.' (New Age International Publishers Hyderabad, India).

Arunakanthi, B., Srinivasprasad, M., Madhanmohan, K., Balachandran, S., Madhav, M., Reddy, C. and Viraktamath, B. 2008. Introgression of major blast resistance genes Pi-1, Pi-2 and Pi-kh in indica rice cultivars Samba Mahsuri and Swarna. J. Mycol. Plant Pathol 38, 625-630.

Ballini, E., Morel, J.-B., Droc, G., Price, A., Courtois, B., Notteghem, J.-L. and Tharreau, D. 2008. A genome-wide meta-analysis of rice blast resistance genes and quantitative trait loci provides new insights into partial and complete resistance. Mol. Plant-Microbe Interact. 21, 859-868.

Cochran, W.G., Cox, G.M. and Liz, A.C. 1965. 'Experimental designs.' (Trillas: Mexico).

Costanzo, S. and Jia, Y. 2010. Sequence variation at the rice blast resistance gene Pi-km locus: implications for the development of allele specific markers. Plant Sci. 178, 523-530.

Fuentes, J.L., Correa-Victoria, F.J., Escobar, F., Prado, G., Aricapa, G., Duque, M.C. and Tohme, J. 2008. Identification of microsatellite markers linked to the blast resistance gene Pi-1 (t) in rice. Euphytica 160, 295-304.

Gupta, S.K., Rai, A.K., Kanwar, S.S., Chand, D., Singh, N.K. and Sharma, T.R. 2012. The single functional blast resistance gene Pi54 activates a complex defence mechanism in rice. J. Exp. Bot. 63, 757-772.

Hittalmani, S., Parco, A., Mew, T., Zeigler, R. and Huang, N. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor. Appl. Genet. 100, 1121-1128.

Hua, L., Wu, J., Chen, C., Wu, W., He, X., Lin, F., Wang, L., Ashikawa, I., Matsumoto, T. and Wang, L. 2012. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast. Theor. Appl. Genet. 125, 1047-1055.

IRRI 1996. Standard evaluation system for rice. In '. ' 4 edn. pp. 52: International Rice Research Institute, Manila, Philippine).

Joshi, R.K. and Nayak, S. 2010. Gene pyramiding-A broad spectrum technique for developing durable stress resistance in crops. Biotechnology and Molecular Biology Reviews 5, 51-60.

Kalode, M. and Krishna, T. 1979. 'Varietal resistance to brown planthopper in India.'

KAU 2011. Package of practices recommendations: crops. In '. ' 14 edn. pp. 360. (Kerala Agricultural University: Trichur, Kerala, India).

Krishnaveni, D., Laha, G., Srinivas, P., Ladha, L., Mangrauthia, S., Prakasam, V. and Viraktamath, B. 2012. Disease resistance in rice. In '. Vol. 62. pp. 1- 21. (Balaji Scan Private ltd, Hyderabad: Directorate of rice research).

Li, J., Li, D., Sun, Y. and Xu, M. 2012. Rice blast resistance gene Pi1 Identified by MRG4766 marker in 173 Yunnan rice landraces. Rice Genomics and Genetics 3.

Liu, G., Lu, G., Zeng, L. and Wang, G.-L. 2002. Two broad-spectrum blast resistance genes, Pi9 (t) and Pi2 (t), are physically linked on rice chromosome 6. Mol. Genet. Genomics 267, 472-480.

Manilal, K. 1990. Ethnobotany of the rices of Malabar. Contribution to Indian Ethnobotany, 243-253.

Ou, S. 1985. 'Rice Diseases (2nd Edn.) ' (Common Wealth Mycological Institute: Kew, UK).

Prasad, M., Madhav, M., Laha, G., Lakshmi, D., Krishnaveni, D., Mangrauthia, S., Balachandran, S., Sundaram, R., Arunakanthi, B., Mohan, K., Madhavi, K., Kumara, V. and Virakthamath, B. 2011. In 'Rice Blast Disease and its Management. Vol. 57. pp. 12-25. (Balaji Scan Private ltd, Hyderabad: Directorate of Rice Research).

Rai, A.K., Kumar, S.P., Gupta, S.K., Gautam, N., Singh, N.K. and Sharma, T.R. 2011. Functional complementation of rice blast resistance gene Pi-k h (Pi54) conferring resistance to diverse strains of Magnaporthe oryzae. Journal of Plant Biochemistry and Biotechnology 20, 55-65.

Rekha, T., Martin, K.P., Sreekumar, V. and Madassery, J. 2011. Genetic diversity assessment of rarely cultivated traditional indica rice (Oryza sativa L.) varieties. Biotechnology research international 2011.

Reporter 2007. Pests and disease outbreak in rice. In 'The Hindu. ' Kerala, India)

Sharma, T., Rai, A., Gupta, S. and Singh, N. 2010. Broad-spectrum blast resistance gene Pi-kh cloned from rice line Tetep designated as Pi54. Journal of Plant Biochemistry and Biotechnology 19, 87-89.

Tuite, J. 1969. 'Plant pathological methods. Fungi and bacteria.' (Burgess Publishing Co: Minneapolis).

Von Braun, J. 2007. 'The world food situtation: new driving forces and required actions.' (International Food Policy Research Institute: Washington, D.C.).

Yamini, V. 2012. Bio intensive management of blast disease of rice (oryza sativa L.). Kerala Agricultural University, Kerala, India


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