Induction of systemic resistance in chilli (Capsicum annuum L.) by Pseudomonas aeruginosa against anthracnose pathogen Colletotrichum capsici

M.S. Jisha, M.S. Linu, J. Sreekumar

Abstract


Colletotrichum capsici Butler and Bisby, the causal agent of chilli anthracnose disease, sternly affects chilli yield and quality worldwide. Biocontrol agents and resistance inducers could be a valid alternative to chemical pesticides. The capability of two phosphate solubilizing Pseudomonas aeruginosa isolates viz., Ps 2 and Ps 3 (KR270346 and KR270347) against chilli anthracnose both under in vitro and in vivo conditions was demonstrated. Pseudomonas aeruginosa Ps 2 showed maximum mycelial growth inhibition of 93.41% and the isolate Ps 3 showed 72.5% inhibition of Colletotrichum capsici in dual culture inhibition assay after 5 days of incubation. There was a greater reduction of anthracnose infection caused by C. capsici on matured fruits in Pseudomonas aeruginosa treated plants when compared to untreated control. The strain possessed chitinolytic and proteolytic activities, produced HCN, siderophores, and was able to produce salicylic acid at a moderate level. The isolate produced amylase. However both of them are unable to produce lipase. The isolates induced systemic resistance in chilli corroborated with increased levels of phenylalanine ammonia lyase, peroxidase and polyphenol oxidase under greenhouse experiments. The results indicate the potential of the indigenous Pseudomonas aeruginosa isolates as biocontrol agents against chilli anthracnose.


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