Molecular diversity in chakkarakolli (Gymnema sylvestre R. Br.) assessed through isozyme and RAPD analysis

Smita Nair, R. Keshavachandran

Abstract


Eighteen phenotypically and biochemically distinct Gymnema accessions representing different geographical regions of Kerala were characterized using isozymes and RAPD markers. In the isozyme analysis, three enzyme systems viz., malate dehydrogenase, esterase, and RUBISCO were studied. Five out of the eight resolved loci (62.5%) were polymorphic with the number of alleles expressed at the polymorphic loci being 10. RUBISCO activity was monomorphic across the accessions. In the RAPD assay, 123 amplified products were generated using 15 selected random primers. Of these, 90 bands were polymorphic with the total frequency of polymorphic markers as high as 73.2%. Specific bands were obtained for five accessions. Jaccard’s coefficient ranged between 0.72 and 0.85 indicating a moderate level of variability. Cluster analysis of RAPD data using Unweighted Pair Group Method of Arithmetic Averages produced two major clusters and three sub-clusters. Overall, molecular fingerprinting revealed the existence of considerable genetic variations in the Gymnema germplasm collection from Kerala.


Keywords


Poly acrylamide gel electrophoresis; Random amplified polymorphic DNA; Molecular markers

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]