Induction of desiccation tolerance in recalcitrant curry leaf (Murraya koenigii (L.) Sprengel) seed

K. Arulmoorthy, K. Raja, S. Sundareswaran


Curry leaf is an important leafy vegetable cum spice species grown widely in South India. It is mainly propagated by seeds. But, the seeds possess recalcitrant behavior which is desiccation and freezing sensitive. Also, the seeds have very short shelf life. Therefore, experiments were conducted to induce the desiccation tolerance in the seed by seed treatment for better storage. In this regard, the seeds were soaked in different desiccation tolerance induction chemicals viz., sodium nitroprusside, L-arginine and abscisic acid for 18 h and stored under ambient condition. The results showed that the seed germination (7%) was maintained upto 20 days when the seeds were soaked in abscisic acid at 100 ppm for 18 h. However, untreated control seed lost its viability completely at 10th day of storage. 


Curryleaf; recalcitrant seed, desiccation tolerance

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Abdul-Baki, A. and Anderson, J. D. 1973. Vigor determination in soybean seed by multiple criteria1. Crop Sci., 13 (6):630-633.

Alfaro, F., Richard, E, Pamela, A. and Dennis, J. 1996. Effect of abscisic acid, osmolarity and temperature onin vitro development of recalcitrant mango nucellar embryos. Plant Cell, Tissue Organ Cult., 44 (1):53-61.

Alpert, P. 2005. The limits and frontiers of desiccation-tolerant life. Integr. Comp. Biol., 45 (5):685-695.

Arulmoorthy, K., Raja, K. and Sundareswaran, S. 2019. Physiological and biochemical changes in desiccation sensitive curry leaf (Murraya koenigii (L.) Sprengel) seeds. J. Phytol., 11: 38-41.

Arulmoorthy, K., Raja, K. and Sundareswaran, S. 2020. Effect of storage treatments on physiological and anatomical changes in desiccation sensitive curry leaf, Murraya koenigii (L.) Sprengel seeds. Adv. Res., 21(8): 55-67.

Barbedo, C. J. and Cicero, S. M. 2000. Effects of initial quality, low temperature and ABA on the storage of seeds of Inga uruguensis, a tropical species with recalcitrant seeds. Seed Sci. Technol., 28 (3):793-808.

Beardmore, T. and Whittle, C. A. 2005. Induction of tolerance to desiccation and cryopreservation in silver maple (Acer saccharinum) embryonic axes. Tree Physiol., 25 (8):965-972.

Chin, H. F. 1989. Recalcitrant seeds. Food Fertilizer Technol. Centre Extn. Bull., 1-17.

Costa, M. D., Righetti, K., Nijveen, H., Yazdanpanah, F., Ligterink, W., Buitink, J. and Henk W. M. Hilhorst. 2015. A gene co-expression network predicts functional genes controlling the re-establishment of desiccation tolerance in germinated Arabidopsis thaliana seeds. Planta, 242 (2):435-449.

Faria, J.M. R., Davide, L. C., da Silva, E. A. A., Davide, A. C., Pereira, R. C., André A. M. van L. and Henk W. M. Hilhorst. 2006. Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage. Braz. J. Plant Physiol., 18 (4):503-513.

Goldbach, H. 1979. Imbibed storage of Melicoccus bijugatus and Eugenia brasiliensis(E. dombeyi) using abscisic acid as a germination inhibitor. Seed Sci. Technol., 7(1): 403-406.

ISTA. 2013. International Rules for Seed Testing. Bassersdorf: International Seed Testing Association, Switzerland.

Marques, A, Nijveen, H., Somi, C., Ligterink, W. and Hilhorst, H. 2019. Induction of desiccation tolerance in desiccation sensitive Citrus limon seeds. J. Integr. Plant Biol., 6(5):624-628.

Pammenter, N. W. and Berjak, P. 1999. A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Sci. Res., 9 (1):13-37.

Panse, V. G. and Sukhatme. P. V. 1967. Statistical Methods for Agricultural Workers. Indian Council for Agricultural Research, New Delhi, India.

Poulsen, K. M. and Eriksen, E. N. 1992. Physiological aspects of recalcitrance in embryonic axes of Quercus robur L. Seed Sci. Res., 2 (4):215-221.

Presley, J. T. 1958. Relation of protoplast permeability to cotton seed viability and predisposition to seedling disease. Plant Disease Reporter, 42 (7):852- 854.

Raja, K. and Palanisamy, V. 2009. Physiology, vigour and viability of desiccation - sensitive arecanut (Areca catechu L.) seeds influenced by waxing. Seed Sci. Technol., 37(2): 457-463.

Raja, K., Palanisamy, V. and Selvaraju, P. 2001a. Desiccation sensitivity of recalcitrant curryleaf (Murraya koenigii (Linn.) Sprengel) seeds. IPGRI/Danida Forest Seed Centre (Denmark) Newsletter, 9: 27-29.

Raja, K., Palanisamy, V. and Selvaraju, P. 2001 b. Effect of storage treatments and methods on viability of recalcitrant arecanut (Areca catechu L.) seed. Intl. J. Trop. Agric., 19 (1-4): 93-105.

Raja, K., Palanisamy, V. and Selvaraju P. 2003. Desiccation and cryopreservation of recalcitrant arecanut (Areca catechu L.) embryos. FAO/IPGRI Plant Genet. Resour. Newsl., 133: 16-18.

Roberts, E.H. 1973. Predicting the storage life of seeds. Seed Sci. Technol., 1:499-514.

Singh, S., More, P.K. and Mohan, S.M. 2014. Curry leaves (Murraya koenigii Linn. Sprengel) - A miracle plant. Indian J. Sci. Res., 4 (1):46-52.

Stanwood, P. C. 1986. Preservation of recalcitrant seeds. J. Seed Technol., 10 (2):140-141.

Tompsett, P. 1987. Desiccation and storage studies on Dipterocarp seeds. Ann. Appl. Biol., 110 (2):371-379.

Yamaguchi, S. 2008. Gibberellin metabolism and its regulation. Annu. Rev. Plant Biol., 59:225-251.

Yoshida, S., Douglas, A. and James, H. 1971. Laboratory manual for physiological studies of rice. Laboratory manual for physiological studies of rice. IRRI Publication, Philippines.


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