Callus induction and plantlet regeneration in <i>Aegle marmelos</i> (L.) Corr. Using cotyledon explants
Keywords:
Medicinal trees, callus induction, MS mediumAbstract
A protocol was developed for callus induction and shoot regeneration from cotyledon explants of Aegle marmelos (L.) Corr., a medicinal tree. Murashige and Skoog (MS) medium supplemented with benzyl adenine (2.2 μM) and 2,4-dichlorophenoxy acetic acid (2.26 μM) recorded the highest growth score for callus induction and proliferation. Shoot regeneration response from the callus was best on MS medium containing 8.8 μM benzyl adenine and 2.85 μM indole-3- acetic acid. Callus derived shoots were rooted in vitro on MS medium supplemented with 12.3 μM indole-3- butyric acid. The plantlets were acclimatized in sand and transferred to the field.Downloads
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