Callus induction and plantlet regeneration in <i>Aegle marmelos</i> (L.) Corr. Using cotyledon explants

Callus induction and plantlet regeneration in <i>Aegle marmelos</i> (L.) Corr. Using cotyledon explants

Authors

  • Hazeena Meerasahib Sainulabdeen Department of Plantation Crops and Spices, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram 695522, Kerala, India.
  • Sulekha G. R. Department of Plantation Crops and Spices, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram 695522, Kerala, India.

Keywords:

Medicinal trees, callus induction, MS medium

Abstract

A protocol was developed for callus induction and shoot regeneration from cotyledon explants of Aegle marmelos (L.) Corr., a medicinal tree. Murashige and Skoog (MS) medium supplemented with benzyl adenine (2.2 μM) and 2,4-dichlorophenoxy acetic acid (2.26 μM) recorded the highest growth score for callus induction and proliferation. Shoot regeneration response from the callus was best on MS medium containing 8.8 μM benzyl adenine and 2.85 μM indole-3- acetic acid. Callus derived shoots were rooted in vitro on MS medium supplemented with 12.3 μM indole-3- butyric acid. The plantlets were acclimatized in sand and transferred to the field.

Author Biography

Sulekha G. R., Department of Plantation Crops and Spices, College of Agriculture, Kerala Agricultural University, Vellayani, Thiruvananthapuram 695522, Kerala, India.

Associate professor,Deparment of plantation crops and spices,College of Agriculture,vellayani

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Published

26-11-2008

How to Cite

Sainulabdeen, H. M., & R., S. G. (2008). Callus induction and plantlet regeneration in <i>Aegle marmelos</i> (L.) Corr. Using cotyledon explants. Journal of Tropical Agriculture, 46, 79–84. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/196

Issue

Section

Short communications

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