Isolation and regeneration of protoplasts from the mycelium of <i>Fusarium pallidoroseum</i> – a potential biocontrol agent of water hyacinth [<i>Eichhornia crassipes</i> (Mart.) Solms]
Keywords:
Protoplast, isolation, regenerationAbstract
A protocol for isolation of protoplast from the mycelium of Fusarium pallidoroseum, a potential biocontrol agent of water hyacinth, was standardized. Stable and more medium sized protoplasts were obtained from 18 h old mycelium at 3 h after incubation, using 80 mg of lytic enzyme from Trichoderma harzianum, in 4 ml of 0.01 M citrate buffer (pH 5.85) containing 0.6 M KCl and 5 mM DTT. The protoplasts were regenerated in the regeneration medium.Downloads
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