<i>Agrobacterium tumefaciens</i> mediated transformation and regeneration of ginger (<i>Zingiber officinale</i> Rosc.)

<i>Agrobacterium tumefaciens</i> mediated transformation and regeneration of ginger (<i>Zingiber officinale</i> Rosc.)

Authors

  • B. Suma Department of Plantation Crops and Spices, College of Horticulture, Kerala Agricultural University, KAU P.O., Thrissur 680656, Kerala
  • R. Keshavachandran Department of Plantation Crops and Spices, College of Horticulture, Kerala Agricultural University, KAU P.O., Thrissur 680656, Kerala
  • E.V. Nybe Department of Plantation Crops and Spices, College of Horticulture, Kerala Agricultural University, KAU P.O., Thrissur 680656, Kerala

Keywords:

Genetic transformation, b-glucuronidase, GUS expression

Abstract

The absence of seed set in ginger makes conventional breeding methods inapplicable warranting genetic modification through biotechnological means. Agrobacterium tumefaciens strain EHA105/p35SGUSInt, effective in expressing b-glucuronidase activity, was used to standardize the pre-culture of explants, bacterial dilution, and co-cultivation period, besides evaluating the effect of acetosyringone and post cultivation in darkness, and to assess the optimum concentration of kanamycin as selection agent for transformation. Transformants were recovered on selection media containing 100 mg L–1 kanamycin and a combination of 2,4-D 1.0 mg L–1 and BA 0.5 mg L–1, and regenerated in half strength MS media of BA 3.0 mg L–1 and 2,4-D 0.5 mg L–1. Successful transformation was confirmed by histochemical GUS assay and polymerase chain reaction analysis.

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Published

27-11-2008

How to Cite

Suma, B., Keshavachandran, R., & Nybe, E. (2008). <i>Agrobacterium tumefaciens</i> mediated transformation and regeneration of ginger (<i>Zingiber officinale</i> Rosc.). Journal of Tropical Agriculture, 46, 38–44. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/186

Issue

Section

Regular papers

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