Efficient screening of brinjal genotypes for bacterial wilt resistance at seedling stage: Unravelling the mechanisms through biochemical parameters
Keywords:
Ralstonia solanacearum, Brinjal genotypes, Biochemical analysis, Disease resistanAbstract
Brinjal genotypes were screened for resistance to bacterial wilt (BW) caused by Ralstonia solanacearum atthe seedling stage and biochemical parameters were analysed to uncover the underlying resistancemechanisms. Three-week-old seedlings of 31 brinjal genotypes including four resistant checks(RC: Ponny,Haritha, Surya and Neelima) and two susceptible checks [SC:SM-26,(S.incanum),SM-27(S.insanum)] weresubjected to a screening procedure that combined soil drenching and root dip inoculation.Based on percentagedisease incidence (PDI), two accessions SM-15 and SM-25 were rated as highly resistant(HR: 0 PDI) andaccession SM-1 was rated as resistant(R: 1-10PDI). Accessions SM-1, SM-2, SM-5, SM-11, SM-17,SM-24and KAU hybrid Neelima were rated as moderately resistant (MR: 11-20 PDI). Accessions SM-4, SM-8,SM-14 and SM-19 were rated as susceptible (S: 21-30 PDI). Moderately susceptible (MS: 31-40 PDI)accessions were SM-3, SM-32, SM-33, SM-13 and SM-16. Accessions SM-9, SM-10, SM-12, SM-18, SM-20, SM-21, SM-22, SM-23, SM-26 and SM-27 were rated as highly susceptible (HS:>40PDI). Biochemicalanalysis showed higher phenol content, Polyphenol Oxidase (PPO) activity, and total sugars in resistant andmoderately resistant groups at 12 and 24 hours post inoculation (hpi). PPO activity, phenolic and totalsugars had a strong negative correlation with disease incidence at 12 and 24 hpi.References
Alam, I. and Salimullah, M. 2021. Genetic engineering of eggplant (Solanum melongena L.): Progress, controversy and Potential. Hortic. 7(78): 1-3.
Ali, M., Quadir, M.A., Okubo, H. and Fujieda, K., 1990. Resistance of eggplant, its wild relatives and their hybrids to different strains of Pseudomonas solanacearum. Sci. Hort., 45(1-2), pp.1-9.
Bainsla, N.K., Singh, S., Singh, P.K., Kumar, K., Singh, A.K. and Gautam, R.K., 2016. Genetic behaviour of bacterial wilt resistance in brinjal (Solanum melongena L.) in tropics of Andaman and Nicobar Islands of India. Amer. J. Plant Sci. 7 (2)-333-338.
Hamilton, C. D., Steidl, O. R., MacIntyre, A. M., Hendrich, C. G., and Allen, C. 2021. Ralstonia solanacearum depends on catabolism of myo-inositol, sucrose, and trehalose for virulence in an infection stage–dependent manner. Mol. Plant-Microbe Interactions 34(6): 669-679.
Hussain, M.Z., Rahman, M.A., and Bashar, M.A. 2005. Screening of brinjal accessions for bacterial wilt caused by Ralstonia solanacearum. Bangladesh J. Bot., 34(1): 53-58.
Kavitha, R. and Umesha, S., 2008. Regulation of defense-related enzymes associated with bacterial spot resistance in tomato. Phytoparasit. 36: 144-159.
Liu, Y. H., Song, Y. H., and Ruan, Y. L.2022. Sugar conundrum in plant–pathogen interactions: roles of invertase and sugar transporters depend on pathosystems. J. Exp. Bot. 73(7): 1910-1925.
Lowe-Power, T. M., Hendrich, C. G., von Roepenack-Lahaye, E., Li, B., Wu, D., Mitra, R., Dalsing, B. L., Ricca, P., Naidoo, J., Cook, D., Jancewicz, A., Masson, P., Thomma, B., Lahaye, T., Michael, A. J., and Allen, C. 2018. Metabolomics of tomato xylem sap during bacterial wilt reveals Ralstonia solanacearum produces abundant putrescine, a metabolite that accelerates wilt disease. Environ. Microbiol. 20(4):1330-1349.
Matsuoka, T. K., Toyota, K., Sato, K., Masuda, and Kuroda, T. 2005. Suppressive mechanisms of used pumice to bacterial wilt of tomato. Soil Microorg., 59: 15–20.
Mishra, B. B. and Sharma, A. 2012. Purification and characterisation of polyphenol oxidase (PPO) from eggplant (Solanummelongena). J. Food Chem. 134(4): 1855-1861.
Mahadevan, A. and Sridhar, R. 1986. Methods In Physiological Plant Pathology (3rd Ed.). Sivakami publications, Chennai, pp. 192-193.
Maharijaya, A., Oktavia, D., Giyanto, G., Harti, H. and Darma, K., 2022, December. Resistance Test of 6 Eggplant (Solanum melongena Linn.) Genotype Against Bacterial Wilt Disease (Ralstonia solanacearum) in the Greenhouse and on the Field. In International Symposium Southeast Asia Vegetable 2021,pp. 484-494.
Niranjan, R. S., Sarosh, B. R., Shetty, H. S. 2006. Induction and accumulation of polyphenol oxidase activities as implicated in development of resistance against pearl millet downy mildew disease. Funct. Plant Biol. 33: 563–571.
Naseem, M., Kunz, M., and Dandekar, T. 2017. Plant–pathogen maneuvering over apoplastic sugars. Trends Plant Sci. 22: 740–743.
O’Brien, J.A., Daudi, A., Butt, V.S. and Paul Bolwell, G., 2012. Reactive oxygen species and their role in plant defence and cell wall metabolism. Planta, 236, pp.765-779.
Pommerrenig, B., Müdsam, C., Kischka, D., Neuhaus, H. E. 2020. Treat and trick: common regulation and manipulation of sugar transporters during sink establishment by the plant and the pathogen. J. Exp. Bot. 71: 3930–3940.
Prakasha, A. and Umesha, S. 2016. Biochemical and molecular variations of guaiacol peroxidase and total phenols in bacterial wilt pathogenesis of Solanum melongena. Biochem. Anal. Biochem. 5(3): 1-7.
Sadasivam, S. and Manickam, A. 1996. Biochemical Methods (3rdedn). New Age International (P) Ltd., New Delhi. 2(4): 272p.
Singh, N., Phukan, T., Sharma, P. L., Kabyashree, K., Barman, A., Kumar, R., Sonti, R. V., Genin, S., and Ray, S. K. 2018. An innovative root inoculation method to study Ralstonia solanacearum pathogenicity in tomato seedlings. Phytopathol. 108(4): 436-442.
Thilagavathi, R., Saravanakumar, D., Ragupathi, N., and Samiyappan, R. 2007. A combination of biocontrol agents improves the management of dry root rot (Macrophominaphaseolina) in greengram. Phytopathol. Mediterranean 46: 157–167.
Vanitha, S. C., Niranjana, S. R., and Umesha, S. 2009. Role of phenylalanine ammonia lyase and polyphenol Oxidase in host resistance to bacterial wilt of tomato. J.Phytopathol. 157: 552-557.
Vasse, J., Fray, P., and Trigalet, A. 2005. Microscopic infection of intercellular infection and protoxylem invasion of tomato roots by Pseudomonas solanacearum. Mol. Plant microbe Inter. 8: 241-251.
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