Distribution of Stunted Disease of Black Pepper in Kerala: Varietal Response and Sero-molecular Characterization of PYMoV
Keywords:
Piper yellow mottle virus (PYMoV), Cucumber mosaic virus(CMV), Black pepper, ELISA, PCRAbstract
Study on viruses infecting black pepper indicated that infection of Cucumber mosaic virus (CMV) and Piper yellow mottle virus (PYMoV) either alone or as mixed infections was prevalent in cultivated areas of Idukki and Wayanad districts of Kerala. Highest disease incidence (DI) was observed in Poothadi (45.50 %) of Wayanad and lowest in Karunapuram (21.62 %) of Idukki district. Highest vulnerability index (V.I.) was recorded in Panamaram (61) of Wayanad and lowest in Pampadumpara (32) of Idukki. Serological technique DAS-ELISA was used for detection of these viruses along with molecular detection using coat protein specific primers. The isolates of PYMoV from different locations ofIdukki and Wayanad showed similarity in ORF III to other reported PYMoV sequences.References
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