Distribution of Stunted Disease of Black Pepper in Kerala: Varietal Response and Sero-molecular Characterization of PYMoV

Distribution of Stunted Disease of Black Pepper in Kerala: Varietal Response and Sero-molecular Characterization of PYMoV

Authors

  • Arya M Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Vellayani 695 522, Kerala, India
  • Radhika N.S. Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Vellayani 695 522, Kerala, India
  • Joy M. Coconut Research Station, Balaramapuram 695 501, Kerala, India
  • Heera G. Department of Plant Pathology, College of Agriculture, Kerala Agricultural University, Vellayani 695 522, Kerala, India
  • Beena R. Department of Plant Physiology, College of Agriculture, Kerala Agricultural University, Vellayani 695 522, Kerala, India

Keywords:

Piper yellow mottle virus (PYMoV), Cucumber mosaic virus(CMV), Black pepper, ELISA, PCR

Abstract

Study on viruses infecting black pepper indicated that infection of Cucumber mosaic virus (CMV) and Piper yellow mottle virus (PYMoV) either alone or as mixed infections was prevalent in cultivated areas of Idukki and Wayanad districts of Kerala. Highest disease incidence (DI) was observed in Poothadi (45.50 %) of Wayanad and lowest in Karunapuram (21.62 %) of Idukki district. Highest vulnerability index (V.I.) was recorded in Panamaram (61) of Wayanad and lowest in Pampadumpara (32) of Idukki. Serological technique DAS-ELISA was used for detection of these viruses along with molecular detection using coat protein specific primers. The isolates of PYMoV from different locations ofIdukki and Wayanad showed similarity in ORF III to other reported PYMoV sequences.

References

Ayisha, R. 2010.Characterisation and management of viral diseases of Black pepper (Piper nigrum L.) Ph.D. (Ag.) Thesis, Kerala Agricultural University, 115 p.

Bhat, A.I., Devasahayam, S., Sarma, Y. R., and Pant, R. P. 2005a. Association of a Badnavirus in black pepper (Piper nigrum L.) transmitted by mealy bug (Ferrisia virgata) in India. Curr. Sci. 84: 1547-1550.

Bhat, A.I., Devasahayam, S., Venugopal, M.N., and Suseelabhai, R. 2005b. Distribution and incidence of viral diseases of black pepper (Piper nigrum L.) in Kerala and Karnataka. Indian J. Plant. Crops 33: 16-21.

Bhat A.I., Hareesh, P.S., and Thomas, T. 2003. Viral diseases of some important spice crops: Vol 2. Characterization, Diagnosis, and Management of viral diseases in Horticultural crops. Studium Press, LLC USA, pp. 223- 248.

Bhat, A.I., Siju, A., Jiby, M.V., Thankamani, C.K. , and Mathew, P.A. 2009. Polymerase Chain Reaction (PCR) based indexing of Black pepper (Piper nigrum L.) plant against Piper yellow mottle virus. J. Spices Aromat. Crops 18(1): 28-32.

Bos, L., 1982. Crop losses caused by viruses. Crop Protection 1(13):263-268.

Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15.

Niblett , C. L., and Claflin, L. E. 1978. Corn lethal necrosis- a new virus disease of corn in Kansas. Plant Dis. Rep. 62:15-19.

Pio-Ribeiro, G., Wyatt, S. D., and Kuhn, C. W. 1978. Cowpea stunt: A disease caused by a synergistic interaction of two viruses. Phytopathol. 68:1260-1265.

Sarma, Y.R., Kiranmai G., Sreenivasulu P., Anandaraj M., Hema M., Venkatramana M., Murthy A.K., and Reddy, D.V.R., 2001. Partial characterization and identification of a virus associated with stunt disease of black pepper (Piper nigrum) in South India. Curr. Sci. 80: 459-462.

Selvarajan, R., Balasubramanian, V., Jeyabaskaran, K.J., Pandey, S.D. and Mustaffa, M.M. 2009. Management of banana bract mosaic disease through higher dose of fertilizer in banana cv. Ney Poovan (AB). Indian J. Hortic. 66 (33): 301-305.

Umadevi, P., Bhat, A. I., Krishnamurthy, K. S. and Anandaraj, M. 2016. Influence of temperature on symptom expression, detection of host factors in virus infected Piper nigrum L. Indian J. Exp. Biol. 54(5): 354-360

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Published

01-12-2023

How to Cite

M, A., N.S., R., M., J., G., H., & R., B. (2023). Distribution of Stunted Disease of Black Pepper in Kerala: Varietal Response and Sero-molecular Characterization of PYMoV. Journal of Tropical Agriculture, 61(1), 111–116. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/1337

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