Histology of organogenesis from callus cultures of black pepper (Piper nigrum L.)

Abstract

A histological study of the indirect organogenesis from leaf cultures of black pepper was carried out. The undifferentiated cells of callus were found to differentiate into vascular nodules called meristemoids, which then develop into xylem elements, especially tracheids. On culturing in the shooting medium, these nodules differentiated into shoot apical meristem. This adventitious origin provides chances for variability.