A modified protocol for isolation of high quality total RNA from ginger (Zingiber officinale Rosc.) rhizomes

S. Sreeja, M. R. Shylaja, Deepu Mathew

Abstract


Ginger rhizomes contain high amount of water, polysaccharides, polyphenols and secondary metabolites which interfere with isolation of total RNA. High quality RNA with sufficient quantity is crucial for conducting gene expression studies. An effective protocol for isolation of RNA from ginger rhizomes is essential. The present study is focused on isolation of high quality RNA from ginger rhizome which can be further utilized for downstream applications like cDNA library preparation and differential gene expression studies. The protocol reported by Kumar et al. (2007) was modified and was compared with the original protocol. The modified protocol was found effective in getting higher quality (A260/A280 – 1.95 to 2.05) and quantity (51-58.6 μg/g) of isolated total RNA from fresh and frozen rhizomes. In the original protocol the quality obtained was (A260/A280 - 1.47 to 1.54) and quantity was 24.0-42.0 μg/g.


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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]