Entry, movement and infection of 32P labelled Ralstonia solanacearum (Smith) Yabuuchi et al. in chilli (Capsicum annuum L.)

Baby Lissy Markose, M. Abdul Vahab, K.V. Peter, N.V. Kamalam, James Mathew

Abstract


Autoradiography and radioassay techniques were utilized to study entry, movement, growth and multiplication, colonisation and infection of Ralstonia solanacearum in chilli. Radioassay of the inoculated resistant and susceptible varieties of chilli revealed that radioactivity counts were more in susceptible variety than in the resistant variety labelled with 32P at 1 h as well as 24 h of inoculation. This radiotracer technique using 32P labelled bacteria in chilli is a potential tool in such plant pathological studies.


Keywords


Autoradiography; 32P; radioactivity; radioassay; Ralstonia solanacearum

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]