Genetic characterization of plant growth promoting rhizobacteria of two prominent leguminous trees in western Rajasthan
Keywords:
<i>Acacia Senegal</i>, <i>Prosopis cineraria</i>, Polymorphism, 16S rRNAAbstract
Plant growth promoting rhizobacteria (PGPRs) from the rhizosphere and root nodules of native Acacia senegal (L.) Willd. and Prosopis cineraria (L.) Druce trees in Western Rajasthan were molecularly characterized by direct sequencing of the 16S rRNA gene. Of the nine isolates obtained from A. senegal, the lone rhizospheric isolate was identified as Stephyllococus hominis Kloos and Schleifer and the remaining eight from root nodules were characterised as Sinorhizobium saheli De Lajudie. Eight isolations from P. cineraria included Lysinibacillus sphaericus Meyer Neide and Bacillus subtilis (Ehrenberg) Cohn (one isolate each) from rhizosphere and Sinorhizobium kostiense Nick (one) and S. saheli (five) from root nodules. Eight RAPD primers detected intra-specific variations generating scorable amplicons and revealed 130 bands with 100% polymorphism. The phylogram based on multiple sequence alignment of 16S rRNA gene delineated not only different genera of PGPRs but also recognized four strains of S. saheli.Downloads
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