In vitro clonal shoot morphogenesis of commercial Dendrobium orchid cultivars in polyamines supplemented medium

I. Priya Kumari, T. Sabina George

Abstract




The effects of polyamines, spermine and spermidine in enhancing shoot morphogenesis were evaluated at 0.25, 0.5, and 1 mM in three commercial Dendrobium cultivars: ‘Rungnappa Red’, ‘Miss Snow White’ and ‘Earsakul’. The response was variable among the varieties. In vitro shoot initiation hastened as spermine and spermidine concentration increased from 0.25 to 1 mM in ‘Rungnappa Red’. For example, shoot numbers increased by 116% with spermine and 3 to 100% with spermidine. In ‘Miss Snow White’ also, up to 200% increase in shoot number over the control was noted for spermine 0.5 mM.


Keywords


Shoot proliferation; Spermine; Spermidine

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]