Plant regeneration of Coscinium fenestratum (Gaertn.) Colebr. through axenic seed culture and axillary bud culture
Keywords:
Conservation, Cosciniumfenestratum, Menispermaceae, micropropagation, axillary bud culture, Axenic seed cultureAbstract
In vitro culture methods were studied for Coscinium fenestratum (Gaertn) Colebr. (F. Menispermaceae), a critically endangered and highly traded medicinal plant, for its conservation and mass propagation. Best surface sterilization of seeds was achieved in axenic seed culture using bavistin (0.3%) for 20 min and streptocyclin (0.1%) for 20 min followed by 0.1 per cent HgCl2‚ (15 min). Among the different media tested for in vitro seed germination, the sterilized sand: coir pith (1:1) soaked with distilled water was found to be better with the highest germination percentage (22.95 %) and lowest germination time (55.68 days). An efficient shoot initiation and multiplication protocol was developed using seedling cotyledonary nodal explant in MS medium supplemented with 0.2mg L-1 BA and 0.06 mg L-1 2, 4 - D (4.5 shoots/culture). When nodal segments with an axillary bud from polyhouse grown plants were used as explants, WPMwas found to be better than the MS medium for shoot initiation and among the growth regulators, kinetin at 0.4 mg L-1 was found to be superior for shoot induction (91.63%). Zygotic embryo excised from GAƒ pre-treated seeds (4000 ppm GAƒ solution for 72 hrs.) when cultured on MS medium in dark condition for 2 weeks followed by exposure to the light condition showed faster development of the embryo, radicle emergence (100 %), plumule emergence (77.78 %) and seedling development (44.44 %).References
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