An improved method for rapid propagation of <i>Phalaenopsis</i> hybrids via culture of longitudinally bisected shoot tips.

An improved method for rapid propagation of <i>Phalaenopsis</i> hybrids via culture of longitudinally bisected shoot tips.

Authors

  • L.K. Preetha
  • K.S. Shylaraj
  • P.C. Rohini

Keywords:

Longitudinally bisected, Micropropagation, Phalaenopsis, Physiological age, Protocorm-likebody, Shoot tip

Abstract

A new procedure for in vitro propagation of orchids belonging to the genus Phalaenopsis was developed. In contrast to the commonly employed micropropagation methodsthat make use of dormant flower stalk buds or intact shoot tips, we have used longitudinally bisected shoot tips derived from 3 – 3.5 month old axenic plantlets of Phalaenopsis hybrids for protocorm-like-bodies (PLB) induction. This procedure in combination with the use of New Dogashima Medium for PLB proliferation resulted in large-scale production of plantlets. Culturing the bisected shoot tips in half strength solid Murashige and Skoog medium supplemented with Thidiazuron (TDZ) (4.54μM–9.08 μM) and 10% coconut water resulted in early PLB induction in 20-25 days and callus-free formation of 13.6 – 15.2 shoot buds in 8 weeks. Intact shoot tips took 6-8 weeks to PLB induction and proliferated to a mean of 5.46 - 7 shoot buds per shoot tip in 12 - 16 weeks in different TDZ concentrations. Continuous multiplication of PLBs and shoot buds was maintained in New Dogashima Medium devoid of hormones by bimonthly subcultivation. About 50,000 hardened plantlets could be produced from a single flowerstalkwith 10 dormant buds, within 2 years from initiation of axenic shoot tip cultures.This protocol can be used to produce genetically stable Phalaenopsis orchids on a commercial scale.

References

Ernst, R. 1994. Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). For. Plant Cell Tiss Organ Cult., 39:273–275.

Gantait, S., Bustam, S. and Sinniah, U.R. 2012. Alginate encapsulation, short-term storage and plant regeneration from protocorm-like bodies of Aranda Wan Chark Kuan 'Blue'× Vanda coerulea Grifft. ex. Lindl. For.Plant Growth Regulat., 68: 129-140.

Gnasekaran, P., Rathinam, X., Sinniah, U.R. and Subramaniam, S. 2010. A study on the use of organic additives on the protocorm-like-bodies(PLBs) growth of Phalaenopsis violaceae orchid. J Phytol., 1 :29-33.

Gnasekaran, P., Poobathy, R., Mahmood, M., Samian, M.and Subramaniam, S. 2012. Effects of complex additives on improving the growth of PLBs of Vanda Kasem’s Delight. AJCS., 6 (8):1245-1248. Genkov, T. and Iordanka, I.1995. Effect of cytokinin-active phenyl urea derivatives on shoot multiplication, peroxidise and super oxide dismutase activities of

in-vitro cultured carnation. Bulgarian J Plant Physiol., 21:73-83.

Liao, Y.J., Tsai, Y.C., Sun, Y.W., Lin, R.S. and Wu, F.S. 2011. In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant., 47:702-709.

Lin,C.C. 1987. Histological observations on in-vitro formation of protocorm-like bodies from flower stalk internodes of Phalaenopsis. Lindleyana., 2 (1): 58-65.

Malabadi, R.B., Mulgund, G.S. and Nataraja, K. 2004. Efficient regeneration of Vanda coerulea, an endangered orchid using thidiazuron. Plant Cell Tissue Organ Cult., 76:289–293.

Mondal, T., Aditya, S. and Banerjee, N. 2013. In vitro axillary shoot regeneration and direct protocorm-like body induction from axenic shoot tips of doritis pulcherrima Lindl. Plant Tissue Cult. & Biotech.,. 23 (2): 251-261.

Murashige, T. and Skoog, F.1962. A revised medium forrapid growth and bioassays with tobacco tissue cultures. Physiol Plant., 15: 473-497.

Murthy, H.N. and Pyati, A.N. 2001. Micropropagation of Aerides maculosum Lindl. (Orhidaceae). In Vitro Cell. Dev Biol Plant., 37: 223-226.

Samarfard, S., Kadir, M., Kadzimin M. Ravanfar, S. and Saud, H. 2013. Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like-bodies as affected by chitosan. Not Bot Horti Agrobo., 4(1):177-183.

Sujjaritthurakarn, P. and Kanchanapoom, K. 2011. Efficient Direct Protocorm-Like Bodies Induction of Dwarf Dendrobium using Thidiazuron. Not Sci Biol., 3 [4]:88-92.

Tokuhara, K. and Mii, M.1993. Micropropagation of Phalaenopsis and Doritaenopsis by culturing shoot tips of flower stalk buds. Plant Cell Rep., 13: 7-11.

Udomdee, W., Pei-Jung, W., Shih-Wen, C. and Fure-Chyi, C. 2012. Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods. African J Biotech., 11 (76):14077-14082.

Zimmer, K. and Pieper, W. 1975. Weitere Untersuchungen zur Kultu in vitro von Aechmea. Gartenbau uwissenschaft., 40: 129–132

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Published

03-11-2017

How to Cite

Preetha, L., Shylaraj, K., & Rohini, P. (2017). An improved method for rapid propagation of <i>Phalaenopsis</i> hybrids via culture of longitudinally bisected shoot tips. Journal of Tropical Agriculture, 55(1), 45–51. Retrieved from https://jtropag.kau.in/index.php/ojs2/article/view/459

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