<i>In vitro </i> clonal shoot morphogenesis of commercial <i>Dendrobium </i>orchid cultivars in polyamines supplemented medium
Keywords:
Shoot proliferation, Spermine, SpermidineAbstract
The effects of polyamines, spermine and spermidine in enhancing shoot morphogenesis were evaluated at 0.25, 0.5, and 1 mM in three commercial Dendrobium cultivars: ‘Rungnappa Red’, ‘Miss Snow White’ and ‘Earsakul’. The response was variable among the varieties. In vitro shoot initiation hastened as spermine and spermidine concentration increased from 0.25 to 1 mM in ‘Rungnappa Red’. For example, shoot numbers increased by 116% with spermine and 3 to 100% with spermidine. In ‘Miss Snow White’ also, up to 200% increase in shoot number over the control was noted for spermine 0.5 mM.Downloads
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