Gluconic acid production as the principal mechanism of mineral phosphate solubilization by <i>Burkholderia </i>sp. (MTCC 8369)
Keywords:
Plant growth-promoting bacteria, 16S rDNA typing, FAME Analysis, SiderophoreAbstract
Eighty one potential phosphate solubilizing bacteria (PSB) were isolated from the rhizosphere soils of crop plants in Athirampuzha, Kottayam, Kerala and were screened for their efficiency. Burkholderia sp. (MTCC 8369) emerged as the most efficient isolate based on biochemical characteristics, FAME analysis, and 16S rDNA typing (GQ246871). The conditions for maximum P solubilisation were: pH 7, temperature 32.5ºC, 18 days of incubation, glucose as a source of carbon, ammonium sulphate as N source, 0.5–1% salt, and 3% sugar. HPLC analysis of the culture filtrate identified gluconic acid as the principal organic acid released by Burkholderia sp. The isolate also exhibited antifungal activity and produced indole acetic acid (IAA), siderophore, hydrogen cyanide, and exopolysacharides. This isolate with its multidimensional plant growth promotion characteristics broadens the spectrum of phosphate solubilizers for field application.Downloads
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