Impact of auxins and activated charcoal on in vitro rooting of Dendrobium chrysotoxum Lindl. cv. Golden Boy

Saikat Gantait, N. Mandal, P.K. Das

Abstract


A protocol was developed for in vitro rooting of Dendrobium chrysotoxum Lindl. cv. Golden Boy where different auxin sources [indole-3-butyric acid (IBA), -naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA)] were evaluated in the presence of activated charcoal (AC). A combination of 0.2 mg•dm–3 IBA plus 2 g•dm–3 AC in MS proved excellent in terms of earliness in root induction, root number, and length as well as response to rooting of in vitro generated microshoots. IBA performance was better than NAA or IAA in presence of AC for root induction and elongation.


Keywords


Auxin sources; <i>In vitro</i> rhizogenesis.

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]