Development of protocorm-like bodies and shoots in Dendrobium cv. Sonia following gamma irradiation

V.L. Sheela, S. Sarada, S. Anitha

Abstract


This paper reports a preliminary attempt to evolve mutant varieties of Dendrobium with desirable traits. Dendrobium cv. Sonia was subcultured on half-strength MS medium supplemented with BA 1.0 mg L-1 and NAA 1.0 mg L-1 to induce production of protocorm-like bodies (PLBs). The PLBs were exposed to different doses of gamma rays (20 Gy and 30 Gy) and re-irradiated from a cobalt 60 source. The highest number of shoots was observed at 20 Gy (3.4). A comparison of three levels of BA with ½ MS media on the irradiated protocorms showed more PLB production at 0.5 mg L-1 while shoot production was higher at 1.5 mg L-1.


Keywords


In vitro mutagenesis; Additive effects

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A KAU publication [CODEN: JTAGEI; ISSN 0971-636X; eISSN 0973-5399]